Resumo: O objetivo deste trabalho foi avaliar o desempenho reprodutivo de jundiá (Rhamdia quelen) alimentado com dietas contendo sorgo em substituição ao milho, suplementadas ou não com fitase. O experimento foi realizado com 360 jundiás, com peso médio inicial de 16,02±0,58 g, alimentados com dietas compostas por 0, 50 e 100% de sorgo de baixo tanino, em substituição ao milho, com ou sem a suplementação de fitase (1.500 UFA por quilograma da dieta). Dietas com suplementação de fitase ou com 100% de sorgo reduziram a deposição de gordura visceral e a massa hepática nos machos. Nas fêmeas, o mesmo efeito foi observado quanto às dietas com suplementação de fitase. As fêmeas alimentadas com dietas apenas com sorgo, suplementadas ou não com fitase, apresentaram ovócitos maiores. Foram encontrados machos e fêmeas nas fases finais de desenvolvimento e maturação gonadal, em todos os tratamentos. A substituição do milho pelo sorgo não afeta a qualidade seminal nem a taxa de fertilização dos gametas. Portanto, a substituição completa do milho pelo sorgo de baixo tanino pode ser efetuada sem afetar os parâmetros reprodutivos e os índices somáticos do jundiá.

Abstract: The objective of this work was to evaluate the reproductive performance of silver catfish (Rhamdia quelen) fed diets containing sorghum, as a replacement for corn, supplemented or not with phytase. The experiment was carried out with 360 fish, with 16.02±0.58 g mean initial weight, fed diets in which low-tannin sorghum replaced corn at 0, 50, and 100%, with or without phytase supplementation (1,500 FTU per kilogram of feed). Diets supplemented with phytase or with 100% sorghum led to a reduction of visceral fat deposition and hepatic mass in males. In females, similar results were observed for diets containing phytase. Females fed sorghum-only diets, supplemented or not with phytase, had bigger oocytes. Males and females at the developing and final stages of gonadal maturation were found in all treatments. The replacement of corn by sorghum affects neither semen quality nor the fertilization rate of gametes. Therefore, complete replacement of corn with low-tannin sorghum in the diet can be performed without compromising the reproductive performance or the somatic indexes of silver catfish.

ABSTRACT Brycon hilarii, popularly called piraputanga in Brazil, is a species distributed throughout the whole basin of the river Paraguay. In recent years, the species has been on a repopulation program due to its remarkable decline as a wild species in the region. Assessment of the genetic diversity of broodstock and fingerling stocks in repopulation programs is basic to avoid genetic impacts on wild populations. The genetic variability of the wild population and of the broodstock and fingerling stocks of B. hilarii in a repopulation program in the river Itiquira MT Brazil will be determined. Seven microsatellite loci produced 52 polymorphic alleles and heterozygosity revealed rates between 0.5794 and 0.7204. FIS did not register any endogamy in the broodstock but it was present in fingerlings and wild populations. Intra- and inter-specific genetic variability rates were higher within each combination but not between groups. Grouping in fingerling groups had a lower density when compared to the others. There is a higher genetic proximity between the natural population and broodstock (0.0237) when the distance between populations was analyzed, even though the two were greatly distant from the fingerling group (0.2622 - 0.2617). Results show that the wild population and the broodstock had high genetic variability and low genetic divergence; contrastingly, fingerlings showed mild genetic variability and great divergence when compared to other groups, indicating that they were not adequately constituted.

O objetivo deste trabalho foi avaliar o efeito de diferentes crioprotetores na viabilidade de embriões de dourado (Salminus brasiliensis). Foram testadas dez soluções crioprotetoras. Para cada solução, 300 embriões foram selecionados na fase do fechamento do blastóporo, e mais 300 embriões foram utilizados como controle negativo. Após o resfriamento (-8ºC por 6 horas), os embriões foram reidratados diretamente na incubadora até a eclosão. O melhor resultado é obtido com solução crioprotetora com 9% de metanol associado a 17% de sacarose, o que resultou em taxa de eclosão de larvas de 67,06%.

The objective of this work was to evaluate the effect of different cryoprotectants on the viability of dourado (Salminus brasiliensis) embryos. Ten cryoprotectant solutions were tested. For each solution, 300 embryos were selected at the closing of the blastopore stage, and 300 more embryos were used as a negative control. After cooling (-8ºC for 6 hours), the embryos were rehydrated directly in the incubator until hatching. The best result is obtained with the cryoprotectant solution containing 9% methanol associated with 17% sucrose, resulting in a larvae hatching rate of 67.06%.